Related Experiment Video
Updated: Jun 27, 2026
10:13
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
Published on: May 6, 2019
Cutting edge: the Idd3 genetic interval determines regulatory T cell function through CD11b+CD11c- APC.
Ana C Anderson1, Rucha Chandwaskar, David H Lee
1Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. aanderson@rics.bwh.harvard.edu
Journal of Immunology (Baltimore, Md. : 1950)
|November 20, 2008
Summary
The Idd3 gene interval protects against autoimmune diseases by influencing regulatory T cells (Tregs). However, this protection is mediated by antigen-presenting cells (APCs), not directly by T cells.
Area of Science:
- Immunology
- Genetics
- Autoimmune Diseases
Background:
- The Idd3 genetic interval provides protection against autoimmune conditions like type 1 diabetes.
- Interleukin-2 (IL-2) is a candidate gene within Idd3, affecting T cell regulation and regulatory T cells (Tregs).
- Previous research indicated that Tregs from Idd3-protected mice (NOD.Idd3) exhibit enhanced suppressive function compared to those from susceptible mice (NOD).
Purpose of the Study:
- To investigate the underlying mechanisms responsible for the differential suppressive activity observed in NOD.Idd3 Tregs compared to NOD Tregs.
- To determine whether the Idd3 genetic interval's protective effect on T cells or antigen-presenting cells (APCs) is responsible for enhanced Treg suppression.
Main Methods:
- Comparative analysis of Treg suppressive activity between NOD and NOD.Idd3 mouse models.
- Investigation of the role of antigen-presenting cells (APCs), specifically CD11b(+)CD11c(-) populations, in modulating Treg function.
- Dissection of immune cell interactions to identify the cellular compartment responsible for differential Treg suppression.
Main Results:
- The enhanced suppressive capacity of NOD.Idd3 Tregs is not intrinsic to the T cells themselves.
- Antigen-presenting cells (APCs) expressing CD11b and CD11c(-) are identified as the key modulators of Treg suppressive function.
- Differences in the APC compartment, rather than T cells, account for the differential suppressive activity of Tregs.
Conclusions:
- The protective effect of the Idd3 genetic interval against autoimmune diseases is mediated through alterations in the antigen-presenting cell (APC) compartment.
- Contrary to initial expectations, the Idd3 locus influences Treg suppression indirectly via APCs, highlighting a complex regulatory network in autoimmunity.
- Understanding the role of APCs in Idd3-mediated protection offers new therapeutic targets for autoimmune diseases.
Related Concept Videos
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:24T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...