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Updated: Jun 27, 2026
Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
Published on: November 10, 2023
Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from
Han-Wen Guo1, Chien-Tsun Chen, Yau-Huei Wei
1Institute of Biophotonics, National Yang-Ming University, 155 Li-Nong Street, Section 2, Taipei 11221, Taiwan.
Abstract:
The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.
