Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chronic Inflammation: Introduction01:12

Chronic Inflammation: Introduction

Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Inflammation: Introduction01:28

Inflammation: Introduction

Inflammation is a fundamental, protective biological response of vascularized tissues to cellular injury, infection, or harmful stimuli. Its primary function is to eliminate the initial cause of injury, clear necrotic cells and damaged tissue, and initiate the necessary repair processes.Cardinal SignsAcute inflammation presents with classic signs. Redness results from vasodilation and increased blood flow. Heat is due to increased metabolism and circulation. Swelling results from the...
Inflammation01:38

Inflammation

Overview
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Acute Inflammation II: Cellular Phase01:26

Acute Inflammation II: Cellular Phase

The cellular phase of acute inflammation is a tightly orchestrated sequence of events that recruits leukocytes, primarily neutrophils, to sites of tissue injury or infection. Following the initial vascular changes, this phase ensures effective immune cell migration, activation, and function at the affected site to eliminate pathogens and initiate tissue repair.Leukocyte Recruitment CascadeLeukocyte recruitment happens in four steps: margination, adhesion, transmigration, and chemotaxis. Reduced...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deletion of Lymphatic PD-L1 Protects Mice From Severe Autoimmune Encephalitis.

European journal of immunology·2026
Same author

uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells.

The Journal of cell biology·2026
Same author

Bioengineered Lymphatic Vessels in Synthetic Matrices to Study Breast Cancer Cell Functions.

Advanced healthcare materials·2026
Same author

Transcriptomics- and 3D imaging-based characterization of the lymphatic vasculature in human skin.

The Journal of experimental medicine·2025
Same author

Lymph nodes fuel KLF2-dependent effector CD8<sup>+</sup> T cell differentiation during chronic infection and checkpoint blockade.

Nature immunology·2025
Same author

Combinatorial treatment with upadacitinib abrogates systemic toxicity of a tumor-targeted IL-2 fusion protein.

Journal for immunotherapy of cancer·2025

Related Experiment Video

Updated: Jun 27, 2026

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies
09:03

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Chapter 1. Inflammation, angiogenesis, and lymphangiogenesis.

Cornelia Halin1, Michael Detmar

  • 1Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland.

Methods in Enzymology
|November 22, 2008
PubMed
Summary

Vascular remodeling, including blood and lymphatic vessel growth, is crucial in chronic inflammatory diseases. Mouse models and methods like immunofluorescence help study these inflammation-induced (lymph)angiogenic responses in vivo.

More Related Videos

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay
09:16

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
09:03

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

Related Experiment Videos

Last Updated: Jun 27, 2026

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies
09:03

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay
09:16

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
09:03

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

Area of Science:

  • Immunology
  • Vascular Biology
  • Pathology

Background:

  • Angiogenesis and lymphangiogenesis are vital for tumor growth and metastasis, with ongoing clinical interventions.
  • Vascular remodeling in chronic inflammatory disorders like psoriasis and rheumatoid arthritis is an emerging therapeutic target.
  • Animal models are essential for studying inflammation-induced vascular changes in vivo.

Purpose of the Study:

  • To review current knowledge on inflammation-induced angiogenesis and lymphangiogenesis.
  • To highlight relevant mouse models for studying these processes.
  • To describe methods for analyzing vascular remodeling in inflamed tissues.

Main Methods:

  • Review of existing literature on inflammation-induced (lymph)angiogenesis.
  • Description of selected mouse models mimicking inflammatory diseases.
  • Detailed explanation of techniques such as immunofluorescence and FACS for quantitation.

Main Results:

  • Summarizes current understanding of inflammation-driven vascular and lymphatic vessel growth.
  • Identifies and discusses key animal models for in vivo research.
  • Outlines methods for visualizing and quantifying vascular remodeling.

Conclusions:

  • Inflammation-induced angiogenesis and lymphangiogenesis are critical in chronic inflammatory diseases.
  • Appropriate animal models and analytical methods are crucial for advancing research in this field.
  • Targeting vascular remodeling offers a promising therapeutic avenue for inflammatory conditions.