Related Experiment Video
Updated: Jun 27, 2026
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
Expression of soluble triggering receptor expression on myeloid cells-1 in pleural effusion
Lu-Ying Huang1, Huan-Zhong Shi, Qiu-Li Liang
1Institute of Respiratory Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, China.
Background:
Triggering receptors expressed on myeloid cells (TREM) proteins are a family of cell surface receptors expressed broadly by cells of the myeloid lineage. The aim of this study was to investigate the clinical significance of soluble TREM-1 (sTREM-1) in pleural effusions, and to determine the effects of pneumonia on pleural sTREM-1 concentrations.
Methods:
Pleural fluid was collected from 109 patients who presented to the respiratory institute (35 with malignant pleural effusion, 31 with tuberculous pleural effusion, 21 with bacterial pleural effusion, and 22 with transudate). The concentrations of sTREM-1, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were determined in effusion and serum samples by enzyme linked immunosorbent assay (ELISA).
Results:
The concentrations of sTREM-1 in bacterial pleural effusion were significantly higher than those in malignant, tuberculous, and transudative groups (all P < 0.001). An sTREM-1 cutoff value of 768.1 ng/L had a sensitivity of 86% and a specificity of 93%. Pleural sTREM-1 levels were positively correlated with levels of TNF-alpha and IL-1beta. Patients with complicating bacterial pneumonia did not have elevated concentration of sTREM-1 in pleural effusion when compared with patients without pneumonia.
Conclusions:
Determination of pleural sTREM-1 may improve the ability of clinicians to differentiate pleural effusion patients of bacterial origin from those with other etiologies. The occurrence of bacterial pneumonia did not affect pleural sTREM-1 concentrations.
Insights
Soluble TREM-1 (sTREM-1) in pleural effusions can help differentiate bacterial infections from other causes. Pleural sTREM-1 levels did not change with bacterial pneumonia.
Area of Science:
- Immunology
- Pulmonology
- Biochemistry
Background:
- Triggering receptors expressed on myeloid cells (TREM) are cell surface receptors on myeloid cells.
- Soluble TREM-1 (sTREM-1) is a biomarker of interest in various inflammatory conditions.
Purpose of the Study:
- To investigate the clinical significance of sTREM-1 in pleural effusions.
- To determine if pneumonia affects pleural sTREM-1 concentrations.
Main Methods:
- Collected pleural fluid from 109 patients with various pleural effusion types.
- Measured sTREM-1, TNF-alpha, and IL-1beta concentrations using ELISA.
- Analyzed sTREM-1 levels in relation to effusion etiology and pneumonia presence.
Main Results:
- Bacterial pleural effusions showed significantly higher sTREM-1 concentrations than other types (P < 0.001).
- An sTREM-1 cutoff of 768.1 ng/L achieved 86% sensitivity and 93% specificity.
- Pleural sTREM-1 correlated positively with TNF-alpha and IL-1beta.
- Bacterial pneumonia did not elevate pleural sTREM-1 levels.
Conclusions:
- Pleural sTREM-1 determination can aid in distinguishing bacterial pleural effusions.
- Bacterial pneumonia occurrence does not influence pleural sTREM-1 concentrations.
More Related Videos
Related Concept Videos
01:25Pleural Effusion I: Introduction
There are two main types of pleural effusion: transudative and exudative. They are differentiated using Light's criteria,...
01:28Pleural Effusion II: Symptoms and Management
A pleural effusion is the abnormal collection of fluid between the parietal and visceral pleura layers of tissue that form the lining of the lungs and chest cavity. It can occur independently or due to surrounding parenchymal diseases, such as infection, malignancy, or inflammatory conditions.
Clinical Manifestations: