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'Gastro' of Mirpur Khas (Sindh) is resolved
Abdul Hannan1, Mahwish Raja, Muhammad Usman Arshad
1Department of Microbiology, University of Health Sciences Lahore, Pakistan. zilufa@hotmail.com
Background:
Diarrhoeal disease is a common cause of major public health concern in many parts of the world including Pakistan.
Methods:
Eighteen stool samples were received from Civil Hospital Mirpur Khas (Sindh). All the specimens were processed for culture and antimicrobial susceptibility according to Clinical Laboratory Standards Institute (CLSI) guidelines.
Results:
Eight out of eighteen (44.4%) samples were positive for Vibrio cholerae. The isolates were gram negative rods, showed darting motility and were Oxidase positive. Contact with distilled water immobilized all these strains (Mdw). API 20NE was used for the biochemical identification and serological confirmation was done with 'difco' antisera. Kirby-Bauer disc diffusion method was performed for their respective susceptibility to various antibiotics. All these isolates were confirmed to be Vibrio cholerae O1 Biotype El Tor Serotype Ogawa. The isolates were generally sensitive to majority of the antibiotics but resistant to nalidixic acid except one strain. Six out of eight isolates were resistant to co-trimoxazole.
Conclusion:
Cholera must be suspected in outbreaks of such kind of 'gastro'.
Insights
Diarrhoeal disease is a significant public health issue. This study identified Vibrio cholerae O1 in 44.4% of samples, highlighting the need for cholera surveillance.
Area of Science:
- Microbiology
- Public Health
- Infectious Diseases
Background:
- Diarrhoeal disease poses a significant public health challenge globally, particularly in regions like Pakistan.
- Prompt identification and management are crucial for controlling outbreaks.
Purpose of the Study:
- To identify the causative agent of diarrhoeal disease outbreaks in Mirpur Khas, Sindh, Pakistan.
- To determine the antimicrobial susceptibility patterns of identified bacterial isolates.
Main Methods:
- Processing of eighteen stool samples using standard microbiological culture techniques.
- Antimicrobial susceptibility testing performed according to Clinical Laboratory Standards Institute (CLSI) guidelines.
- Biochemical identification using API 20NE and serological confirmation with specific antisera.
Main Results:
- Eight out of eighteen (44.4%) samples yielded Vibrio cholerae.
- All confirmed isolates were identified as Vibrio cholerae O1, Biotype El Tor, Serotype Ogawa.
- Isolates showed general sensitivity to most antibiotics but resistance to nalidixic acid and co-trimoxazole in most cases.
Conclusions:
- Cholera should be strongly suspected in outbreaks presenting with gastrointestinal symptoms.
- Effective surveillance and timely diagnosis are essential for managing cholera outbreaks.
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