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Updated: Jun 27, 2026
Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
Published on: February 23, 2017
[Effect of silver-hydroxyapatite/titania nanocomposites on proliferation, vitality and ultrastructure of osteoblasts]
Jing-chao Zhang1, An-chun Mo, Hong-kun Wu
1Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China.
Objective:
Osteoblasts behaviors were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg-HA/TiO2) in vitro.
Methods:
Rat calvaria osteoblasts were cultured in the presence of 10 mirog/mL-500 mirog/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope (TEM).
Results:
Both cell proliferation and vitality were restricted by addition of nanoparticles, while the respiration rates as an index for unit cell viability were enhanced. nAg-HA/TiO2 at low concentration showed superiority. No remarkable ultrastructural changes were observed in the osteoblasts exposed to nanoparticles. The differences in cell proliferation and cell vitality between nAg-HA/TiO2 group and nHA group at the same concentration were insignificant.
Conclusion:
nAg-HA/TiO2 restricted cells proliferation, but triggered unit cell viability. Cytocompatibility of nAg-HA/TiO2 is almost the same as that of nHA's.
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