Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phloem and Sugar Transport02:02

Phloem and Sugar Transport

Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic cells that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic tissues to other specialized tissues by a process called translocation.
Short-distance Transport of Resources02:12

Short-distance Transport of Resources

Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
The Apoplast and Symplast01:46

The Apoplast and Symplast

Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport01:23

Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport

Drugs need to permeate cell membranes to reach their target sites after administration. Orally administered drugs must transcend intestinal epithelial membrane barriers to infiltrate the systemic circulation. Drugs with a molecular weight of less than 500 Daltons diffuse through gaps between neighboring cells, called paracellular pathways.
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...
Transcellular Transport of Solutes01:23

Transcellular Transport of Solutes

Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Crucial Roles of Phloem Companion Cells in Response to Phosphorus Deficiency.

Plant, cell & environment·2025
Same author

Candidatus Liberibacter asiaticus accumulation in the phloem inhibits callose and reactive oxygen species.

Plant physiology·2022
Same author

Delayed Leaf Senescence by Upregulation of Cytokinin Biosynthesis Specifically in Tomato Roots.

Frontiers in plant science·2022
Same author

The Phloem as an Arena for Plant Pathogens.

Annual review of phytopathology·2022
Same author

Assessing Rates of Long-distance Carbon Transport in <i>Arabidopsis</i> by Collecting Phloem Exudations into EDTA Solutions after Photosynthetic Labeling with [<sup>14</sup>C]CO<sub>2</sub>.

Bio-protocol·2021
Same author

Assessing Long-distance Transport from Photosynthetic Source Leaves to Heterotrophic Sink Organs with [<sup>14</sup>C]CO<sub>2</sub>.

Bio-protocol·2021

Related Experiment Video

Updated: Jun 27, 2026

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Phloem transport: cellular pathways and molecular trafficking.

Robert Turgeon1, Shmuel Wolf

  • 1Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA. ert2@cornell.edu

Annual Review of Plant Biology
|November 26, 2008
PubMed
Summary

Phloem sap sampling is challenging due to sieve tube damage. This review covers sampling methods, transport pathways, and mobile compounds like proteins and RNA in vascular plants.

More Related Videos

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
07:14

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

Related Experiment Videos

Last Updated: Jun 27, 2026

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
07:14

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

Area of Science:

  • Plant Biology
  • Plant Physiology
  • Molecular Biology

Background:

  • The phloem is crucial for transporting nutrients, defensive compounds, and signals in vascular plants.
  • Sampling phloem sap is difficult due to the pressurized nature of sieve tubes, often causing damage and artifacts.
  • Understanding phloem transport is vital for plant communication and development.

Purpose of the Study:

  • To review current methods for sampling phloem sap.
  • To discuss artifacts introduced by sampling procedures.
  • To explore the complex pathways and compounds transported within the phloem, including macromolecules.

Main Methods:

  • Literature review of phloem sampling techniques.
  • Analysis of artifacts associated with different sampling methods.
  • Examination of plasmodesmatal conductivity and transporter roles in phloem loading/unloading.

Main Results:

  • Phloem sap sampling methods can introduce artifacts.
  • Phloem transport involves intricate pathways for nutrients, defensive compounds, and signals.
  • Mobile proteins and RNA are key components of long-distance plant communication.

Conclusions:

  • Effective phloem sap sampling remains a challenge.
  • Macromolecular trafficking, including proteins and RNA, plays a significant role in plant signaling.
  • Future research should focus on dissecting the molecular and cellular mechanisms of long-distance macromolecular transport.