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Related Experiment Video

Updated: Jun 27, 2026

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Published on: May 13, 2019

A three-dimensional model of vasculogenesis.

Mani T Valarmathi¹, Jeffrey M Davis, Michael J Yost

¹Department of Cell and Developmental Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina 29209, USA. valarmathi.thiruvanamalai@uscmed.sc.edu

|November 26, 2008

Summary

Bone marrow stem cells (BMSCs) differentiate into vascular cells on a 3-D scaffold, forming capillary-like structures. This in vitro model aids in studying postnatal neovascularization and developing engineered tissues.

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Last Updated: Jun 27, 2026

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

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The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

Published on: November 2, 2016

Area of Science:

Biomedical Engineering
Stem Cell Biology
Vascular Biology

Background:

Postnatal bone marrow harbors stem and progenitor cells capable of vascular differentiation.
These cells can contribute to neovascularization in vivo and in vitro.

Purpose of the Study:

To investigate the differentiation potential of rat bone marrow stem cells (BMSCs) into vascular lineages.
To evaluate a 3-D tubular scaffold for inducing BMSC differentiation and supporting microvessel formation.
To establish an in vitro model for studying postnatal vasculogenesis and engineered tissue perfusion.

Main Methods:

Seeding rat BMSCs onto a 3-D scaffold of aligned type I collagen fibers.
Culturing cells in vasculogenic or non-vasculogenic media for up to 28 days.
Assessing cell differentiation using real-time PCR for transcript levels and confocal microscopy for protein expression.
Analyzing construct morphology via scanning electron microscopy.

Main Results:

BMSCs differentiated into endothelial and smooth muscle cell lineages on the scaffold.
Upregulation of vascular phenotypic markers observed at both transcript and protein levels.
Formation of microvascular capillary-like structures and smooth-walled tube-like structures with associated smooth muscle cells/pericytes.
Endothelial cells demonstrated high acetylated low-density lipoprotein metabolism.

Conclusions:

The 3-D culture system effectively induces BMSC maturation and differentiation into vascular cell lineages.
The system supports microvessel morphogenesis, creating a functional in vitro vascular network.
This model serves as a valuable platform for studying de novo vasculogenesis and addressing perfusion limitations in tissue engineering.