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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

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Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
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Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Single-cell RT-PCR cDNA subtraction

Ebrahim Sakhinia1, Damian L Weaver, César Núñez

  • 1School of Biological Sciences, University of Manchester, Manchester, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 26, 2008
PubMed
Summary

No abstract available in PubMed .

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