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Updated: Jun 27, 2026
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
[Determination of plasma escitalopram with liquid chromatography-mass spectrometry]
Heng Zheng1, Ling Yang, Wen-Yan Tang
1Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. hzheng@tjh.tjmu.edu.cn
Objective:
To establish an liquid chromatography-mass spectrometry (HPLC-MS/MS)-based method for determining escitalopram in human plasma.
Methods:
A liquid-liquid ether extraction approach was adopted using dextromethorphan as the internal standard. The Agilent ZORBAX SB-C18 (3.5 microm,2.1 x 150 mm) was used as the analytical column with acetonitrile-NH4AC buffer (70:30, V/V) as the mobile phase at the flow rate of 0.3 ml/min. The sample was ionized by electrospray ionization source in the triple quadruple tandem mass spectrometer, and the plasma escitalopram determined with a multiple reaction monitoring mode of m/z325.4-->109.4.
Results:
The linear range was 0.0866-64.13 microg/L (r=0.9965) for escitalopram in human plasma, with the absolute recovery between 64.98% and 78.72% and the within-day and between-day deviations less than 10%.
Conclusion:
The method is sensitive, accurate, rapid, specific and well applicable for clinical pharmacokinetics study of escitalopram.
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