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Updated: Jun 27, 2026
Seven Steps to Stellate Cells
Published on: May 10, 2011
[Effect of genistein on hepatic stellate cell proliferation and lipid peroxidation in vitro]
Hong-Yang Shi1, Jun-Wang Xu, Xiao-Xia Ren
1First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Objective:
To investigate the effect of genistein on the proliferation and lipid peroxidation of hepatic stellate cells (HSC) in vitro and its the protective effect against hepatic fibrosis.
Methods:
Rat hepatic stellate cells (HSC-T6 cells) were divided into 3 groups and incubated in the presence of 0.1 mol/L hydrogen dioxide followed by washing with PBS for 3 times. Genistein at different concentrations was added into the cell culture meclia, and after 48 h of incubation, the cell proliferation was assessed with MTT assay and the levels of superoxide dismutase (SOD), malonaldehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH-PX) in the supernatant of the cell culture were measured.
Results:
Genistein at different concentrations inhibited the cell proliferation, showing a dose-effect relationship. Genistein significantly decreased the production of intracellular MDA and GSH and increased SOD and GSH PX activity.
Conclusion:
Genistein can prevent the formation of hepatic fibrosis probably by decreasing HSC proliferation and lipid peroxidation.
