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Updated: Jun 27, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Biosurfactant production by Azotobacter chroococcum isolated from the marine environment
R Thavasi1, V R M Subramanyam Nambaru, S Jayalakshmi
1Department of Chemical and Biological Sciences, Polytechnic Institute of New York University, Six Metrotech Center, Brooklyn, NY 11201, USA. hydrobact@rediffmail.com
Abstract:
Preliminary characterization of a biosurfactant-producing Azotobacter chroococcum isolated from marine environment showed maximum biomass and biosurfactant production at 120 and 132 h, respectively, at pH 8.0, 38 degrees C, and 30 per thousand salinity utilizing a 2% carbon substrate. It grew and produced biosurfactant on crude oil, waste motor lubricant oil, and peanut oil cake. Peanut oil cake gave the highest biosurfactant production (4.6 mg/mL) under fermentation conditions. The biosurfactant product emulsified waste motor lubricant oil, crude oil, diesel, kerosene, naphthalene, anthracene, and xylene. Preliminary characterization of the biosurfactant using biochemical, Fourier transform infrared spectroscopy, and mass spectral analysis indicated that the biosurfactant was a lipopeptide with percentage lipid and protein proportion of 31.3:68.7.
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