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High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
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Identification and expression pattern of zebrafish prox2 during embryonic development.

Anna Pistocchi1, Silvia Bartesaghi, Franco Cotelli

  • 1Department of Biology, Università degli Studi di Milano, Milano, Italy.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|November 28, 2008
PubMed
Summary

Prox2 is a zebrafish gene crucial for development, homologous to Drosophila prospero. Its expression is observed in the central nervous system and eyes during embryonic stages and in adult organs.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Comparative Genomics

Background:

  • Prox2 is a vertebrate homolog of the Drosophila homeobox gene prospero.
  • Prox1 and Prox2 are transcription factors vital for developmental events.
  • Understanding Prox2 function is key to deciphering developmental pathways.

Purpose of the Study:

  • To isolate and characterize the zebrafish Prox2 homolog.
  • To investigate the expression pattern of zebrafish prox2 during embryonic and adult stages.
  • To explore potential regulatory interactions between Prox1 and Prox2.

Main Methods:

  • cDNA isolation and sequencing.
  • Comparative genomic analysis to identify syntenic regions.
  • Whole-mount in situ hybridization for embryonic expression analysis.

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Related Experiment Videos

Last Updated: Jun 27, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

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Published on: October 19, 2013

Dissection of Larval Zebrafish Gonadal Tissue
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Published on: April 26, 2017

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
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Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

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  • Reverse transcriptase-polymerase chain reaction for adult organ expression analysis.
  • Prox1 loss- and gain-of-function assays.

    • Main Results:

    • A cDNA encoding a putative zebrafish Prox2 protein with high homology to mammalian Prox1/Prox2 and zebrafish Prox1 was isolated.
    • Comparative genomics confirmed the identified gene as the zebrafish Prox2 homolog, syntenic with mouse Prox2.
    • Embryonic prox2 expression was detected in specific central nervous system and eye structures.
    • Prox2 expression was also found in several adult zebrafish organs.
    • Prox1 manipulation was assessed for its effects on prox2 expression.

    Conclusions:

    • The study successfully identified and characterized the zebrafish Prox2 gene.
    • Zebrafish Prox2 exhibits a conserved expression pattern in the central nervous system and eye during development.
    • Prox2 is expressed in various adult organs, suggesting broader roles.
    • Further investigation into Prox1-Prox2 regulatory relationships is warranted.