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Published on: January 13, 2017
Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis
Wen-Rong Hong1, Wun-Rong Hong, Mei Ge
1College of Biological Science and Technology, Fuzhou University, 523 Gongye Rd, Fuzhou City, Fujian, China.
Abstract:
Micromonospora inyoensis produces sisomicin (Sm), an aminoglycoside antibiotic. The gene cluster of sisomicin biosynthesis spanning ca. 47 kb consists of 37 ORFs encoding various proteins for sisomicin biosynthesis, regulation, resistance and transport. The comparative genetic studies on the biosynthetic genes of sisomicin and gentamicin (Gm) reveal a similar biosynthetic route and provide a framework for the future biosynthetic studies.
Insights
Micromonospora inyoensis produces the antibiotic sisomicin (Sm). Comparative genetic studies reveal a similar biosynthetic route to gentamicin (Gm), aiding future research.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Sisomicin (Sm) is an aminoglycoside antibiotic produced by Micromonospora inyoensis.
- Aminoglycoside antibiotics are crucial for treating bacterial infections.
Purpose of the Study:
- To elucidate the genetic basis of sisomicin biosynthesis.
- To compare the sisomicin biosynthetic gene cluster with that of gentamicin (Gm).
Main Methods:
- Bioinformatic analysis of the sisomicin biosynthetic gene cluster.
- Comparative genomics of sisomicin and gentamicin biosynthetic pathways.
Main Results:
- Identified a 47 kb gene cluster containing 37 open reading frames (ORFs) involved in sisomicin biosynthesis, regulation, resistance, and transport.
- Demonstrated significant similarities between the sisomicin and gentamicin biosynthetic gene clusters and pathways.
Conclusions:
- The genetic framework for sisomicin biosynthesis has been established.
- Comparative analysis provides insights into conserved mechanisms of aminoglycoside antibiotic production and offers a foundation for future biosynthetic engineering and drug discovery efforts.
