Related Experiment Video
Updated: Jul 15, 2026
09:03
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
Published on: October 16, 2017
Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging
M van Lohuizen1, S Verbeek, B Scheijen
1Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
Cell
|May 31, 1991
Summary
Moloney murine leukemia virus (Mo-MLV) infection accelerates B cell lymphomagenesis in E mu-myc mice. Provirus tagging identified four cooperating oncogenes: pim-1, bmi-1, pal-1, and bla-1.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- E mu-myc transgenic mice are models for B cell lymphomagenesis.
- Moloney murine leukemia virus (Mo-MLV) infection accelerates tumor development in these mice.
- Identifying cooperating genes is crucial for understanding B cell transformation.
Purpose of the Study:
- To identify genes cooperating with the E mu-myc transgene in B cell transformation using provirus tagging.
- To characterize novel proviral insertion sites and their encoded proteins.
Main Methods:
- Provirus tagging in E mu-myc transgenic mice infected with Mo-MLV.
- Analysis of proviral insertion sites in pre-B cell lymphomas.
- Bioinformatic analysis of the bmi-1 gene and its protein product.
Main Results:
- Four common proviral insertion loci (pim-1, bmi-1, pal-1, bla-1) were identified in 35%, 35%, 28%, and 14% of tumors, respectively.
- bmi-1, pal-1, and bla-1 represent novel common proviral insertion sites.
- The bmi-1 gene encodes a conserved nuclear protein with transcriptional regulator motifs, including a zinc finger.
Conclusions:
- Provirus tagging is effective for identifying cooperating oncogenes in lymphomagenesis.
- The identified loci, particularly bmi-1, pal-1, and bla-1, are significant in B cell transformation.
- Further research is needed to assign genes to pal-1 and bla-1 and to elucidate their roles.
Related Concept Videos
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...