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Trichinella spiralis--a potential anti-tumor agent
1Department of Pharmacology, Bethune Basic Medicine Faculty, Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, 5333 Xian Road, Changchun, PR China.
Abstract:
Murine forestomach carcinoma (cell line MFC), ascitic hepatoma (cell line H22) and sarcoma (cell line S180) solid tumor models were used to test the anti-tumor effect of Trichinella spiralis in vivo. Mice previously infected by oral administration of 400 viable T. spiralis larvae per mouse for 7 days were grafted with various solid tumor cell lines. Other groups of tumor-bearing mice were given caudal vein injection of crude extracts of adult and newborn larvae at 17.5, 35.0 or 70.0 mg kg(-1). These treatments to inhibit tumor growth were dose-dependent (p<0.05). The anti-proliferative activity of crude T. spiralis extract was examined in vitro at 0.035, 0.070 or 0.140 mg ml(-1) using MFC, H22, S180, human chronic myeloid leukemia cell line (K562) and hepatoma cell line (H7402), tumor cell proliferation in vitro was measured by methyl thiazolium stain and was inhibited in dose-dependent manner (p<0.05). At the same doses, crude T. spiralis extracts induced apoptosis of K562 and H7402 as detected by DNA fragmentation. Cell cycle analysis indicated that crude T. spiralis extracts, at 0.140 mg ml(-1), arrested the cell cycle of K562 and H7402 in G1 or S phase. It is concluded that T. spiralis contains anti-tumor active agent.
Insights
Trichinella spiralis extracts demonstrated significant anti-tumor effects in vivo and in vitro. These findings suggest Trichinella spiralis contains potent anti-cancer agents for therapeutic development.
Area of Science:
- Parasitology
- Immunology
- Oncology
Background:
- Trichinella spiralis is a parasitic nematode.
- The potential anti-tumor properties of parasitic organisms are an area of growing research interest.
Purpose of the Study:
- To investigate the anti-tumor effects of Trichinella spiralis extracts against various cancer cell lines in vitro and in vivo.
- To determine the dose-dependent effects and underlying mechanisms of action.
Main Methods:
- In vivo studies utilized murine models (MFC, H22, S180) treated with T. spiralis larvae or crude extracts.
- In vitro studies assessed crude T. spiralis extract effects on MFC, H22, S180, K562, and H7402 cell lines using proliferation assays and apoptosis detection (DNA fragmentation).
- Cell cycle analysis was performed on K562 and H7402 cells treated with T. spiralis extracts.
Main Results:
- T. spiralis treatments significantly inhibited tumor growth in vivo in a dose-dependent manner.
- Crude T. spiralis extracts exhibited dose-dependent anti-proliferative activity against all tested cancer cell lines in vitro.
- Extracts induced apoptosis and caused cell cycle arrest in G1 or S phase in K562 and H7402 cells.
Conclusions:
- Trichinella spiralis possesses significant anti-tumor activity.
- The findings support the presence of anti-tumor active agents within T. spiralis, warranting further investigation for therapeutic applications.
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