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Related Experiment Videos

The mouse IgH 3'-enhancer.

P Dariavach1, G T Williams, K Campbell

  • 1MRC Laboratory of Molecular Biology, Cambridge, GB.

European Journal of Immunology
|June 1, 1991
PubMed
Summary
This summary is machine-generated.

Researchers identified a conserved lymphoid-specific transcription enhancer element near the rat and mouse immunoglobulin heavy chain (IgH) genes. This enhancer, flanked by inverted repeats, shows stage-specific activity in lymphocyte differentiation.

Related Experiment Videos

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • A lymphoid-specific transcription enhancer element was identified at the 3' end of the rat immunoglobulin heavy chain (IgH) locus.
  • Sequence analysis revealed this enhancer is flanked by a 350-bp inverted repeat, suggesting a transposable element-like structure.

Purpose of the Study:

  • To screen for and characterize the equivalent enhancer in mice to assess evolutionary conservation.
  • To investigate the activity and stage-specific role of the IgH 3'-enhancer during lymphocyte differentiation.
  • To determine the functional impact of the mouse IgH 3'-enhancer on mu gene expression.

Main Methods:

  • Sequence analysis to identify homologous enhancer elements in mice.
  • Screening for the enhancer's presence and flanking repeat structures in the mouse genome.
  • Assessing enhancer activity in various cell lines (plasmacytoma, B cell lymphomas, HeLa cells).
  • Transfection experiments using mu gene expression plasmids to evaluate enhancer function.

Main Results:

  • A mouse homologue of the IgH 3'-enhancer was identified 16 kb downstream of the C alpha 1 exon, also flanked by inverted repeats.
  • The mouse and rat enhancers exhibit high sequence homology.
  • The IgH 3'-enhancer demonstrated lymphoid-specific activity in plasmacytoma lines but not in B cell lymphomas or HeLa cells.
  • Inclusion of the mouse IgH 3'-enhancer increased mu mRNA levels in stable plasmacytoma transfectants.

Conclusions:

  • The IgH 3'-enhancer is conserved between rats and mice and possesses a unique structure resembling a transposable element.
  • Its activity appears to be stage-specific during lymphocyte differentiation, potentially playing a role in later stages like plasmacytoma development.
  • The enhancer contributes to, albeit modestly, the regulation of mu gene expression within the IgH locus.