Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A new synthetic functionalized antigen carrier.

J W Drijfhout¹, W Bloemhoff

¹Department of Medical Microbiology, State University, Groningen, The Netherlands.

International Journal of Peptide and Protein Research

|January 1, 1991

Summary

Researchers developed a novel synthetic antigen carrier using branched lysine residues. This carrier enables high-density antigen conjugation, creating potent immunogenic conjugates for potential applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Non-contact induction heating and SAAP-148 eliminate persisters within MRSA biofilms mimicking a metal implant infection.

European cells & materials·2021

Same author

Antibodies and B cells recognising citrullinated proteins display a broad cross-reactivity towards other post-translational modifications.

Annals of the rheumatic diseases·2020

Same author

H1N1 hemagglutinin-specific HLA-DQ6-restricted CD4+ T cells can be readily detected in narcolepsy type 1 patients and healthy controls.

Journal of neuroimmunology·2019

Same author

Prevention of Staphylococcus aureus biomaterial-associated infections using a polymer-lipid coating containing the antimicrobial peptide OP-145.

Journal of controlled release : official journal of the Controlled Release Society·2015

Same author

CD8 T cell autoreactivity to preproinsulin epitopes with very low human leucocyte antigen class I binding affinity.

Clinical and experimental immunology·2012

Same author

Structure elucidation of gluten-derived peptides by tandem mass spectrometry.

Methods in molecular medicine·2011

Area of Science:

Bioconjugation Chemistry
Immunology
Synthetic Chemistry

Background:

Development of effective antigen carriers is crucial for enhancing immunogenicity.
Existing carriers may have limitations in antigen loading capacity and conjugation efficiency.

Purpose of the Study:

To describe a new synthetic functionalized antigen carrier.
To demonstrate its utility in creating high-molecular-weight conjugates with high antigen content.

Main Methods:

Synthesis of a branched lysine core structure.
Functionalization of lysine residues with N-(S-acetylmercaptoacetyl)-glutamyl groups.
Deprotection to yield thiol groups for conjugation.
Conjugation to a functionalized peptide antigen (e.g., S-(Npys)-cysteinyl peptide).

Main Results:

A novel synthetic antigen carrier with a branched lysine core was successfully synthesized.
The carrier possesses eight thiol functions after deprotection, enabling efficient conjugation.
High molecular weight conjugates with exceptionally high antigen content were achieved.

Conclusions:

The described synthetic antigen carrier offers a versatile platform for creating potent immunogenic constructs.
This approach allows for high-density antigen presentation, potentially improving immune responses.

Related Experiment Videos