Related Experiment Video
Updated: Jun 27, 2026
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Advancements in plant proteomics using quantitative mass spectrometry
Silke Oeljeklaus1, Helmut E Meyer, Bettina Warscheid
1Medizinisches Proteom-Center, Zentrum fuer klinische Forschung, Ruhr-Universitaet Bochum, Bochum, Germany.
Journal of Proteomics
|December 4, 2008
Summary
Quantitative mass spectrometry (MS) advances plant proteomics, enabling detailed analysis of protein changes. This review highlights MS-based methods for understanding protein function, dynamics, and modifications in plants.
Area of Science:
- Plant biology
- Proteomics
- Biochemistry
Background:
- Proteomics has advanced beyond static snapshots to dynamic monitoring of protein changes.
- Quantitative mass spectrometry (MS) technologies are increasingly applied in plant biology.
- Stable isotope labeling and label-free methods are key for protein quantification.
Purpose of the Study:
- To review the latest trends and applications in MS-based quantitative plant proteomics.
- To provide insights into available quantitative MS technologies for plant studies.
- To illustrate the potential of quantitative MS in analyzing plant protein functions.
Main Methods:
- Review of recent literature on MS-based quantitative plant proteomics.
- Discussion of stable isotope labeling techniques.
- Explanation of label-free quantification strategies.
Main Results:
- Quantitative MS enables monitoring of protein abundance, location, and post-translational modifications.
- Applications include comparative and functional proteomics in plants.
- Significant potential shown for organellar and phosphoproteomics, protein dynamics, and turnover.
Conclusions:
- MS-based quantitative proteomics offers powerful tools for in-depth plant functional analysis.
- The technology facilitates large-scale functional characterization of plant proteins.
- Future research can leverage these methods to explore complex plant biological processes.
Related Concept Videos
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:33Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...