Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictive Registry Optimization of Molecular Adsorbates on Solid Surfaces.

Angewandte Chemie (International ed. in English)·2026
Same author

Electrical Control of Intersubband Transitions in Few-Layer WSe<sub>2</sub> Multivalley Quantum Wells Probed by Electronic Raman Scattering.

ACS nano·2026
Same author

Furan-Protected 4-Maleimidomethyl Styrene for Reversible Crosslinked Polymers.

Macromolecular rapid communications·2025
Same author

Structure-Dependent Dynamics of Quantum Emitters in Single-Layer WSe<sub>2</sub>.

Nano letters·2025
Same author

Increased Formation of Trions and Charged Biexcitons by Above-Gap Excitation in Single-layer WSe<sub>2</sub>.

ACS nano·2024
Same author

From π-Conjugated Rods to Shape-Persistent Rings, Wheels, and Ladders: The Question of Rigidity.

Accounts of chemical research·2024

Related Experiment Video

Updated: Jun 27, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Spatial anticorrelation between nonlinear white-light generation and single molecule surface-enhanced Raman

Manfred J Walter1, Nicholas J Borys, Gerald Gaefke

  • 1Department of Physics, University of Utah, Salt Lake City, Utah 84112, USA.

Journal of the American Chemical Society
|December 5, 2008
PubMed
Summary

We found that nonlinear white-light emission and single-molecule surface-enhanced Raman scattering (SERS) on silver films are correlated. Two-photon imaging can identify optimal SERS substrates for single-molecule detection.

More Related Videos

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
12:54

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
12:54

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

  • Plasmonics
  • Nanophotonics
  • Spectroscopy

Background:

  • Surface-enhanced Raman scattering (SERS) enables single-molecule detection.
  • Plasmon-enhanced nonlinear optical phenomena are crucial for advanced spectroscopy.
  • Fractal silver films offer unique plasmonic properties for light-matter interactions.

Purpose of the Study:

  • To investigate the relationship between nonlinear white-light emission and single-molecule SERS.
  • To explore the use of conjugated polymers as analytes for SERS.
  • To assess the potential of two-photon imaging for prescreening SERS substrates.

Main Methods:

  • Utilizing fractal silver films with a conjugated polymer analyte.
  • Employing nonlinear white-light emission measurements under infrared excitation.
  • Performing single-molecule resonance SERS analysis.
  • Applying two-photon imaging techniques.

Main Results:

  • Single molecule SERS was observed at locations without nonlinear light emission.
  • The broad emission background in SERS may not stem from optical field amplifications.
  • Nonlinear emission and SERS capabilities are spatially correlated on fractal films.

Conclusions:

  • Nonlinear white-light emission can serve as an indicator for SERS activity.
  • Two-photon imaging is a promising method for identifying optimal SERS substrates.
  • Understanding emission backgrounds is key to advancing single-molecule SERS.