Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of GS-441524 Derivatives as Potent SARS-CoV-2 Mac1 Inhibitors via a Direct-to-Biology Approach.

bioRxiv : the preprint server for biology·2026

Same author

A ratiometric fluorescent reporter of mitochondrial sodium.

Nature chemical biology·2026

Same author

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026

Same author

SIRT2 mediates integrated stress response by deacetylating and stabilizing 4E-BP1 to suppress translation.

EMBO reports·2026

Same author

Coenzyme A is a redox sensing cofactor for malic enzyme 2 regulating oxidative stress and mitochondrial metabolism.

bioRxiv : the preprint server for biology·2026

Same author

Author Correction: Sirtuin 2 inhibits global protein synthesis via Rheb-GTPase degradation.

EMBO reports·2026

Same journal

Decoding Galectin-Glycan Recognition with <sup>19</sup>F-Tagged Lectins: from Simple Glycans to the Cellular Glycocalyx.

Journal of the American Chemical Society·2026

Same journal

Open- and Closed-Shell Roles of Sensitizer and Annihilator in Pseudo-Single Component Mixtures for Upconversion.

Journal of the American Chemical Society·2026

Same journal

Pressure-Induced Superconductivity at 15 K in van-der-Waals Ferroelectric CuInP<sub>2</sub>S<sub>6</sub>.

Journal of the American Chemical Society·2026

Same journal

Carbene Analogues of Group 15: Reduction of s-Hydrindacene-Based Chloropnictogenium Ions To Access an Antimony Hydride Monocation and a Trinuclear Bismuth Dication.

Journal of the American Chemical Society·2026

Same journal

Chiral-Ligand-Modulated Nickel-Catalyzed Stereoselective Radical Migratory C2-Arylation of Carbohydrates.

Journal of the American Chemical Society·2026

Same journal

Coordination-Constraint-Driven Enhanced Chirality Induction in Perovskite Quantum Dot Solids.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

High-throughput selection for cellulase catalysts using chemical complementation.

Pamela Peralta-Yahya¹, Brian T Carter, Hening Lin

¹Department of Chemistry, Columbia University, New York, New York 10027, USA.

Journal of the American Chemical Society

|December 5, 2008

Summary

This summary is machine-generated.

Developing a high-throughput selection method for cellulase catalysts is crucial for cost-effective biomass to ethanol conversion. This new assay enables rapid screening of enzyme variants, accelerating the discovery of improved glycosylhydrolases for biofuel production.

More Related Videos

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Related Experiment Videos

Last Updated: Jun 27, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Area of Science:

Biotechnology
Enzymology
Biomass Conversion

Background:

Efficient enzymatic hydrolysis of lignocellulosic biomass is key for cost-effective biofuel production.
Current screening methods for glycosylhydrolases limit the improvement of cellulase catalysts.

Purpose of the Study:

To develop and validate the first high-throughput selection for cellulase catalysts.
To adapt chemical complementation for a growth assay detecting bond cleavage reactions.

Main Methods:

Adapted a URA3 counter selection to link chemical dimerizer-activated gene transcription to cell death.
Demonstrated detection of cellulase activity via cleavage of a tetrasaccharide substrate and reduced URA3 reporter expression.
Assessed utility by isolating improved cellulases from a DNA shuffled library.

Main Results:

Successfully developed a high-throughput selection assay for cellulase activity.
Isolated cellulase variants with enhanced activity from a diverse library.
Demonstrated the adaptability of chemical complementation for detecting enzymatic activities.

Conclusions:

The developed high-throughput selection significantly advances cellulase discovery for biomass conversion.
This method has the potential to accelerate the identification of improved glycosylhydrolases.
Chemical complementation is a versatile platform for discovering enzymes for various chemical transformations.