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Updated: Jun 27, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
A ratiometric fluorescent probe for cysteine and homocysteine displaying a large emission shift
Weiying Lin1, Lingliang Long, Lin Yuan
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China. weiyinglin@hnu.cn
Abstract:
4-(1H-phenanthro[9,10-d]imidazol-2-yl)benzaldehyde 1 was rationally designed as a novel ratiometric fluorescent probe for cysteine and homocysteine. Upon addition of cysteine or homocysteine, notably, the probe displayed a very large (125 nm) hypsochromic shift in emission due to switching off intramolecular charge transfer. This large emission wavelength shift may allow probe 1 to be employed for quantitatively detecting Cys/Hcy.

