Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Bacterial Phylum Actinobacteria

Bacterial Phylum Actinobacteria

Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...

Microbial Classification System

Microbial Classification System

Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...

Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimization of cell permeabilization in electron flow based mitochondrial function assays.

Free radical biology & medicine·2021

Same author

GenoBase: comprehensive resource database of Escherichia coli K-12.

Nucleic acids research·2014

Same author

Decreased tryptophan metabolism in patients with autism spectrum disorders.

Molecular autism·2013

Same author

Using phenotype microarrays to determine culture conditions that induce or repress toxin production by Clostridium difficile and other microorganisms.

PloS one·2013

Same author

Assay of the multiple energy-producing pathways of mammalian cells.

PloS one·2011

Same author

Important discoveries from analysing bacterial phenotypes.

Molecular microbiology·2008

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Published on: September 12, 2018

Global phenotypic characterization of bacteria.

Barry R Bochner¹

¹Biolog Inc., 21124 Cabot Boulevard, Hayward, CA 94545, USA. bbochner@biolog.com

FEMS Microbiology Reviews

|December 5, 2008

Summary

Systems biology models are validated by predicting microbial cell behaviors. Phenotype MicroArrays offer a global view of cellular phenotypes, aiding model validation and advancing microbiology research.

Area of Science:

Systems biology
Microbiology
Genomics
Proteomics
Metabolomics
Phenomics

Background:

Systems biology models aim to reproduce and predict biological system behaviors, like microbial cells.
Model sophistication has increased with layered data sets for genomics, proteomics, metabolomics, and phenomics.
This review specifically addresses the validation and application of microbial cell phenotypes.

Purpose of the Study:

To describe the development and utility of Phenotype MicroArrays.
To highlight their role in validating systems biology models.
To explore diverse applications in basic and applied microbiology.

Main Methods:

More Related Videos

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Related Experiment Videos

Last Updated: Jun 27, 2026

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Published on: September 12, 2018

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Review of the development of Phenotype MicroArray technology.

Analysis of data generated from global phenotyping.

Examination of applications in systems biology and microbiology.

Main Results:

Phenotype MicroArrays provide a global perspective on cellular phenotypes.
This technology is crucial for validating and refining systems biology models.
Numerous applications exist for basic and applied microbiology research.

Conclusions:

Phenotype MicroArrays are essential tools for advancing systems biology.
Global phenotyping enhances the accuracy and predictive power of microbial models.
The technology has broad utility across various microbiology research areas.