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Related Experiment Video

Updated: Oct 16, 2025

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Optimization of cell permeabilization in electron flow based mitochondrial function assays.

Xiang-He Lei1, Barry R Bochner1

  • 1Biolog, Inc., 21124 Cabot Blvd., Hayward, CA, 94545, USA.

Free Radical Biology & Medicine
|October 17, 2021
PubMed
Summary
This summary is machine-generated.

Optimizing cell permeabilization is key for mitochondrial research. This study evaluates agents like saponin and digitonin using a novel redox dye assay for improved mitochondrial function studies.

Keywords:
Cell permeabilizationMitochondrial assayMitochondrial functionMitochondrial inhibitorsMitochondrial metabolismMitochondrial substrates

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Mitochondrial Research

Background:

  • Permeable cell models are crucial for advancing mitochondrial research.
  • Effective permeabilization requires selective membrane permeability while preserving organelle integrity and function.

Purpose of the Study:

  • To assess and optimize commonly used permeabilizing agents for cell-based mitochondrial assays.
  • To evaluate the efficacy of saponin, digitonin, and perfringolysin O in maintaining mitochondrial function.

Main Methods:

  • Utilized a novel electron flow-based mitochondrial assay.
  • Employed a colorimetric redox dye for quantitative measurements.
  • Assessed various permeabilizing agents including saponin, digitonin, and recombinant perfringolysin O.

Main Results:

  • The study provides a comparative analysis of different permeabilizing agents.
  • Optimized conditions were determined for using permeabilized cells in mitochondrial assays.
  • The novel redox dye assay demonstrated effectiveness in evaluating mitochondrial function.

Conclusions:

  • This research offers guidance for optimizing cell permeabilization techniques in mitochondrial studies.
  • The findings support the use of the novel redox dye assay for reliable mitochondrial function assessment.
  • Proper agent selection and optimization are critical for successful permeable cell model applications.