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The construction of a monoclonal diagnostic system for the field detection of Vibrio cholerae
A al-Riyami1, L G Haynes, A M Campbell
1Department of Biochemistry, University of Glasgow, U.K.
FEMS Microbiology Immunology
|February 1, 1991
Abstract:
An enzyme-based double monoclonal field diagnostic system detecting both serotypes of Vibrio cholerae has been developed. The system uses nitrocellulose as a solid support, 1.25% skimmed dried milk as blocking reagent, water as washing reagent, and alkaline phosphatase cross-linked to antibody by means of glutaraldehyde as detecting reagent. The sensitivity of the system was 10(5) vibrios/ml. The biotin-avidin system gave sensitivity an order of magnitude weaker. There were no cross-reactions with the range of other bacteria tested.