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[Normal rabbit sera. An immunologic and immunohistologic study].

L Reinhold-Richter1, H Renner

  • 1Forschungsinstitut für Lungenkrankheiten und Tuberkulose Berlin-Buch, BRD.

Zentralblatt Fur Pathologie
|January 1, 1991
PubMed
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Most rabbits possess antimycobacterial antibodies due to environmental exposure. High sensitivity immunohistological techniques like peroxidase-antiperoxidase (PAP) reveal widespread natural immunisation, necessitating careful animal selection for accurate antibody testing.

Area of Science:

  • Immunology
  • Veterinary Pathology
  • Microbiology

Background:

  • Antimycobacterial antibodies in non-immune rabbit sera are crucial for diagnostic test development.
  • High prevalence of natural immunisation can complicate antibody detection studies.
  • Environmental factors, such as cohabitation with fowl, may contribute to mycobacterial antigen exposure.

Purpose of the Study:

  • To investigate the presence and prevalence of antimycobacterial antibodies in non-immune rabbit sera.
  • To compare the sensitivity of different immunological techniques for antibody detection.
  • To identify potential sources of natural immunisation in rabbit populations.

Main Methods:

  • Immunological studies using transmigration electrophoresis.
  • Immunohistochemical analysis employing the peroxidase-antiperoxidase (PAP) technique.

Related Experiment Videos

  • Assessment of rabbit sera for antibodies against mycobacteria.

    • Main Results:

    • Transmigration electrophoresis detected antimycobacterial antibodies in 11% of rabbit sera.
    • The highly sensitive PAP technique identified antibodies in 94% of rabbit sera.
    • Cohabitation with fowl was identified as a potential cause for high antigen contamination.

    Conclusions:

    • The peroxidase-antiperoxidase (PAP) technique demonstrates significantly higher sensitivity in detecting antimycobacterial antibodies compared to transmigration electrophoresis.
    • Widespread natural immunisation against mycobacteria is prevalent in rabbit populations, likely due to environmental antigen exposure.
    • Antimycobacterial antisera and control sera should be sourced from animals with no history of natural immunisation to prevent false-positive results, especially when using sensitive immunohistological methods.