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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Jun 27, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

The antisense transcriptomes of human cells.

Yiping He1, Bert Vogelstein, Victor E Velculescu

  • 1Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA.

Science (New York, N.Y.)
|December 6, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a new technique to identify and quantify RNA transcripts from specific DNA strands. This method revealed pervasive antisense transcripts in human cells, suggesting a key role in gene regulation.

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Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Related Experiment Videos

Last Updated: Jun 27, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Area of Science:

  • Molecular Biology
  • Genomics
  • Gene Expression Analysis

Background:

  • Genome-wide transcription analysis is established, but distinguishing RNA transcript origins (sense vs. antisense) from DNA strands is challenging.
  • Understanding the relationship between sense and antisense transcripts is crucial for elucidating gene regulatory mechanisms.

Purpose of the Study:

  • To develop and validate a novel technique for identifying the DNA strand of origin for RNA transcripts.
  • To globally quantify sense and antisense transcripts from expressed genes in human cells.

Main Methods:

  • A new technique was developed to determine the DNA strand of origin for individual RNA transcripts.
  • The method was applied to globally quantify sense and antisense transcripts across five human cell types.

Main Results:

  • The technique successfully identified the DNA strand of origin for RNA transcripts.
  • Evidence for antisense transcripts was found in 2900 to 6400 human genes across all examined cell types.
  • The distribution of antisense transcripts was distinct from sense transcripts, nonrandom across the genome, and varied among cell types.

Conclusions:

  • Antisense transcripts are a pervasive feature of human cells.
  • Antisense transcripts likely represent a fundamental component of gene regulation in humans.