Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Phosphoenolpyruvate:carbohydrate phosphotransferase systems in Enterococcus faecalis].

A Muraoka1, K Ito, H Nagasaki

  • 1Department of Medical Technology, Kochi Gakuen College.

Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology
|March 1, 1991
PubMed
Summary

Enterococcus faecalis utilizes multiple carbohydrate transport systems. Loss of the constitutive mannose-phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in mutants impacts other PTS activities and carbohydrate metabolism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal change in the physical performance of older adults in the community.

Journal of the Japanese Physical Therapy Association = Rigaku ryoho·2015
Same author

D-Aspartyl residue in a peptide can be liberated and metabolized by pig kidney enzymes.

Amino acids·2013
Same author

Reduction of insulin signaling upregulates angiopoietin-like protein 4 through elevated free fatty acids in diabetic mice.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2011
Same author

Palmitate induces apoptosis in Schwann cells via both ceramide-dependent and independent pathways.

Neuroscience·2010
Same author

Contractile properties of human ankle muscles determined by a systems analysis method for the EMG-force relationship.

Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology·2010
Same author

Protective effect of hedgehog signaling on cytokine-induced cytotoxicity in pancreatic beta-cells.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2010

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Context:

  • Enterococcus faecalis employs phosphoenolpyruvate:carbohydrate phosphotransferase systems (PTSs) for nutrient uptake.
  • Understanding PTS substrate specificity and regulation is crucial for bacterial physiology.

Purpose:

  • To investigate the presence and characteristics of PTSs in wild-type and 2-deoxy-D-glucose (2DG)-resistant Enterococcus faecalis mutants.
  • To determine the relationship between the mannose-PTS and other carbohydrate PTSs in this organism.

Summary:

  • Wild-type E. faecalis has a constitutive mannose-PTS active with glucose, mannose, glucosamine, 2DG, and fructose, which is absent in mutants.
  • Mutants exhibit altered PTS activity, including a potential independent maltose-PTS and inducible lactose-, mannitol-, and trehalose-PTSs.

Related Experiment Videos

  • The inability of mutants to induce lactose-PTS with galactose suggests impaired galactose metabolism upon loss of the mannose-PTS.

    • Impact:

    • This study elucidates the complex interplay between different PTSs in E. faecalis.
    • Findings suggest that the constitutive mannose-PTS plays a role in the regulation or formation of galactose-related metabolites necessary for lactose-PTS induction.