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Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Does antioxidant system adaptive response alleviate related oxidative damage with long term bed rest?
I Margaritis1, A S Rousseau, J F Marini
1Department for evaluation of nutritional and health risks, French Food Safety Agency, Maisons-Alfort, France. i.margaritis@afssa.fr
Clinical Biochemistry
|December 9, 2008
Summary
Long-term bed rest (LTBR) causes oxidative stress, increasing antioxidant enzyme activity. Resistance exercise helps mitigate these effects by preserving enzyme function, with biomarkers returning to normal upon activity resumption.
Area of Science:
- Physiology
- Biochemistry
- Exercise Science
Background:
- Long-term bed rest (LTBR) is known to induce physiological deconditioning.
- Understanding the impact of hypokinesia on oxidative stress is crucial for developing countermeasures.
- Erythrocyte antioxidant enzyme activity changes during prolonged inactivity require further investigation.
Purpose of the Study:
- To assess oxidative damage and erythrocyte antioxidant enzyme activity during LTBR and recovery.
- To evaluate the potential prophylactic effects of resistance exercise against LTBR-induced oxidative stress.
- To investigate the relationship between antioxidant intake and oxidative stress markers during LTBR.
Main Methods:
- 11 healthy males underwent LTBR.
- Nutrient intake was monitored throughout the study.
- Biomarkers were assessed on days 60 and 90 of LTBR, and 90 days post-LTBR.
Main Results:
- LTBR caused a minor decrease in total thiol protein concentrations.
- Glutathione peroxidase activity increased during LTBR and decreased post-recovery, indicating oxidative stress.
- Lipoperoxidative markers remained unchanged, and effects were independent of antioxidant intake.
Conclusions:
- Hypokinesia induces an oxidative stress, evidenced by altered antioxidant enzyme activity.
- Resistance exercise partially alleviates hypokinesia effects, preserving glutathione reductase activity.
- Return to physical activity normalizes oxidative stress biomarkers, highlighting the importance of exercise and nutrition.
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