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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system
Kwang-Wook Ko1, Fengqiu Lin, Takumi Katsumata
1Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
Bioscience, Biotechnology, and Biochemistry
|December 9, 2008
Abstract:
Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.
