Related Experiment Video
Updated: Jun 27, 2026

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
Published on: May 29, 2016
[Identification of a new carbohydrate-binding site of influenza virus]
Abstract:
It has recently been shown that the influenza virus can specifically bind the residue of a nonsialylated sulfated oligosaccharide Gal(6SO(3)H)beta1-4GlcNAcbeta (6'SLacNAc). To identify by photoaffinity labeling the virion component that binds 6'SLacNAc, we synthesized a carbohydrate probe containing a (125)I labeled diazocyclopentadien-2-yl carbonyl group as an aglycone. According to the electrophoretic data, the labeled areas corresponded to a large hemagglutinin subunit, a nucleocapsid protein, and neuraminidase (NA). Probing in the presence of an excess of 6'SLacNAcbeta-OCH(2)CH(2)NHAc glycoside resulted in redistribution of the labeling intensity, with the maximum inhibition being observed for NA. The data obtained indicate that NA is a viral 6'SLacNAc-binding protein.
Related Concept Videos
Inhibitors Of Virion Release
Influenza
Leaky Scanning

