Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Injectable hydrogels incorporating cell-derived nanocarriers for tumor therapy and regenerative medicine.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Biodegradable oligo(amidoamine/β-amino ester) hydrogels for controlled insulin delivery.

Soft matter·2026
Same author

Recent developments and prospects of inorganic nanozymes for biomedical applications.

Biomaterials science·2025
Same author

Bioengineered metastatic cancer nanovaccine with a TLR7/8 agonist for needle-free intranasal immunization.

Biomaterials·2025
Same author

Targeted delivery of anti-miRNA21 sensitizes PD-L1<sup>high</sup> tumor to immunotherapy by promoting immunogenic cell death.

Theranostics·2024
Same author

Stem Cell-Derived Extracellular Vesicle-Bearing Injectable Hydrogel for Collagen Generation in Dermis.

ACS applied materials & interfaces·2024

Related Experiment Video

Updated: Jun 27, 2026

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

Injectable in situ-forming pH/thermo-sensitive hydrogel for bone tissue engineering.

Hea Kyung Kim1, Woo Sun Shim, Sung Eun Kim

  • 1Department of Polymer Science & Engineering, Sungkyunkwan University, Suwon, Republic of Korea.

Tissue Engineering. Part A
|December 9, 2008
PubMed
Summary

A novel injectable hydrogel scaffold was developed for bone tissue engineering. This pH/thermo-sensitive polymer supports human mesenchymal stem cell and bone morphogenetic protein-2 encapsulation, showing potential for in situ bone formation.

More Related Videos

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
10:19

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Published on: August 8, 2022

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
06:05

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells

Published on: July 14, 2023

Related Experiment Videos

Last Updated: Jun 27, 2026

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
10:19

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Published on: August 8, 2022

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
06:05

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells

Published on: July 14, 2023

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Autologous bone tissue engineering requires advanced scaffolds for cell delivery and differentiation.
  • Developing injectable, in situ-forming hydrogels is crucial for minimally invasive bone regeneration.
  • Existing scaffolds often lack the necessary responsiveness to physiological conditions for optimal cell integration.

Purpose of the Study:

  • To synthesize and characterize a novel pH- and thermo-sensitive hydrogel.
  • To evaluate the hydrogel's potential as an injectable scaffold for bone tissue engineering.
  • To assess the hydrogel's ability to support human mesenchymal stem cell (hMSC) differentiation and bone formation in vivo.

Main Methods:

  • Synthesized a pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer.
  • Assessed gelation properties at physiological and altered pH/temperature conditions.
  • Evaluated hydrogel biocompatibility using Dulbecco's modified Eagle's medium extract test.
  • Determined encapsulation efficiencies of hMSCs and recombinant human bone morphogenetic protein-2 (rhBMP-2).
  • Injected the hydrogel with hMSCs and rhBMP-2 into mice to evaluate ectopic bone formation and hMSC differentiation.

Main Results:

  • The synthesized block copolymer formed a stable gel under physiological conditions (pH 7.4, 37°C) and a sol at pH 8.0, indicating injectability.
  • The hydrogel demonstrated high biocompatibility.
  • High encapsulation efficiencies were achieved for hMSCs (~90%) and rhBMP-2 (~85%).
  • In vivo studies showed hMSC differentiation and mineralized tissue formation with alkaline phosphatase activity up to 7 weeks post-injection.

Conclusions:

  • The pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer is a promising injectable scaffold for bone tissue engineering.
  • The hydrogel facilitates in situ gelation and supports cell viability and differentiation.
  • This novel material holds potential for advancing bone regeneration therapies.