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Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

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Related Experiment Video

Updated: Jun 21, 2025

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Stem Cell-Derived Extracellular Vesicle-Bearing Injectable Hydrogel for Collagen Generation in Dermis.

Dong Gil You¹, Jae Min Jung², Chan Ho Kim²

¹Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, United States.

ACS Applied Materials & Interfaces

|July 9, 2024

Summary

A novel stem cell-derived extracellular vesicle (EV)-bearing thermosensitive hydrogel (EVTS-Gel) promotes significant in vivo collagen generation. This advanced dermal filler offers improved anti-aging effects and injection convenience compared to existing treatments.

Keywords:

collagen controlled release dermal filler extracellular vesicles thermosensitive hydrogel

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Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

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Last Updated: Jun 21, 2025

Generation of Self-assembled Vascularized Human Skin Equivalents

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Published on: February 12, 2021

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

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Published on: February 7, 2018

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

Area of Science:

Biomaterials Science
Regenerative Medicine
Dermatology

Background:

Current dermal fillers face limitations in stimulating collagen generation and offer suboptimal injection convenience.
Stem cell-derived extracellular vesicles (EVs) show promise for regenerative applications but require effective delivery systems.

Purpose of the Study:

To develop and evaluate a novel stem cell-derived extracellular vesicle (EV)-bearing thermosensitive hydrogel (EVTS-Gel) for enhanced in vivo collagen generation.
To assess the injection convenience and anti-aging efficacy of the developed EVTS-Gel.

Main Methods:

Development of a thermosensitive hydrogel (TS-Gel) with sol-gel transition at 32.6 °C for controlled release of EVs.
Evaluation of rheological properties and injection forces of the TS-Gel and EVTS-Gel.
Assessment of EV retention time, collagen layer thickness, and inhibition of dermis thickness reduction in mouse skin models over 24 weeks.

Main Results:

The EVTS-Gel demonstrated suitable rheological properties and required lower injection forces.
Local administration of EVTS-Gel extended EV retention time by 2.23 times.
EVTS-Gel significantly inhibited age-related dermis thickness reduction and increased collagen layer thickness by 2.64-fold compared to bare EV treatment, even at a 10× lower dose.

Conclusions:

The developed EVTS-Gel serves as an effective platform for delivering stem cell-derived EVs for in vivo collagen generation.
EVTS-Gel shows significant potential as an advanced anti-aging dermal filler with improved efficacy and delivery characteristics.