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Updated: Jun 27, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Nucleic acids detection using cationic fluorescent polymer based on one-dimensional microfluidic beads array.

Xiaohai Yang1, Xiang Zhao, Xinbing Zuo

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Engineering Center for Biomedicine, Institute of life science and Biotechnology, Changsha, PR China.

Talanta
|December 10, 2008
PubMed
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A new assay uses a cationic fluorescent polymer on a 1-D chip for rapid DNA/mRNA detection. This label-free method simplifies nucleic acid analysis and is cost-effective for multiple targets.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Nucleic acid detection assays often require complex labeling steps.
  • Developing rapid, cost-effective, and label-free methods for DNA and mRNA detection is crucial for molecular diagnostics.

Purpose of the Study:

  • To develop a novel assay for direct and rapid detection of DNA and mRNA using a cationic fluorescent polymer on a 1-D microfluidic chip.
  • To evaluate the assay's performance in detecting specific gene targets and analyzing gene expression changes.

Main Methods:

  • A cationic water-soluble polythiophene derivative was employed as a fluorescent reporter.
  • The polymer was integrated into a one-dimensional microfluidic beads array (1-D chip) for fluorescence detection.
  • The assay was used to detect p53 complementary DNA (cDNA) and quantify mRNA expression of tumor-associated genes (p53, c-myc, cyclin-d1) in cell lines.

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Last Updated: Jun 27, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Main Results:

  • The cationic fluorescent polymer effectively transduced hybridization events into measurable optical signals.
  • The 1-D chip assay demonstrated rapid detection of p53 cDNA across various concentrations.
  • Analysis of mRNA expression in CNE2 cells showed changes in p53, c-myc, and cyclin-d1 levels after 5-fluorouracil (5-FU) treatment, validated by RT-PCR.

Conclusions:

  • A label-free, rapid, and cost-effective assay for DNA/mRNA detection has been successfully developed using a cationic fluorescent polymer on a 1-D chip.
  • This assay simplifies experimental procedures and offers potential for low-cost, multi-target nucleic acid analysis.
  • The system provides a valuable tool for gene expression studies and molecular diagnostics.