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Updated: Jun 27, 2026

Spatial Separation of Molecular Conformers and Clusters
Published on: January 9, 2014
Nanoscale separation of molecular species based on their rotational mobility
Ilaria Testa1, Andreas Schönle, Claas von Middendorff
1Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Abstract:
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.
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