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In vitro stimulation of bovine circulating lymphocytes by parainfluenza type 3 virus
Research in Veterinary Science
|January 1, 1977
Abstract:
Bovine circulating lymphocytes from animals sero-positive to parainfluenza-3 virus (PIV-3) could be stimulated by PIV-3 antigen in a microculture system. A semiautomatic multiple sample harvester was used to collect the lymphocytes from the microplates. Ultraviolet-killed PIV-3 induced better stimulation values than formalin or heat-treated virus. The highest stimulation values were obtained after a stimulation time of three to five days.