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Preparation and Fractionation of Xenopus laevis Egg Extracts
Published on: August 27, 2008
Preparation and fractionation of Xenopus laevis egg extracts
Marie K Cross1, Maureen Powers
1Department of Cell Biology, Emory University, USA. mkcross@emory.edu
Journal of Visualized Experiments : Jove
|December 11, 2008
Abstract:
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
