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Updated: Jun 27, 2026

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In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
Published on: September 2, 2008
In vitro nuclear assembly using fractionated Xenopus egg extracts
1Department of Cell Biology, Emory University, USA. mkcross@emory.edu
Journal of Visualized Experiments : Jove
|December 11, 2008
Abstract:
Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

