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Related Experiment Video

Updated: Jun 27, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Single cell electroporation in vivo within the intact developing brain.

D Sesath Hewapathirane1, Kurt Haas

  • 1Brain Research Centre, University of British Columbia.

Journal of Visualized Experiments : Jove
|December 11, 2008
PubMed
Summary

Single-cell electroporation (SCE) precisely delivers molecules into individual cells in intact tissues. This method enables cell-autonomous studies and live imaging of cellular processes in Xenopus tadpoles.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Single-cell electroporation (SCE) allows targeted molecular delivery into individual cells within intact tissues.
  • This technique distinguishes cell-autonomous effects from global treatment effects.
  • Advanced in vivo imaging combined with SCE enables visualization of cellular dynamics.

Purpose of the Study:

  • To optimize and detail the procedure for single-cell electroporation in Xenopus laevis tadpoles.
  • To demonstrate the delivery of fluorescent markers into individual neurons within the intact tadpole brain.
  • To showcase the utility of SCE for live in vivo imaging of cellular events.

Main Methods:

  • Single-cell electroporation (SCE) of fluorescent dye or plasmid DNA into Xenopus tadpole neurons.

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Related Experiment Videos

Last Updated: Jun 27, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Neonatal Pial Surface Electroporation
06:22

Neonatal Pial Surface Electroporation

Published on: May 7, 2014

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
10:45

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Published on: June 14, 2020

  • Optimization of SCE parameters for improved yield.
  • Live two-photon fluorescence imaging of electroporated neurons.

    • Main Results:

    • Successful delivery of fluorescent markers into individual neurons in the intact Xenopus brain.
    • Demonstration of optimized SCE protocols for high-yield transfection.
    • Visualization of cellular morphology and intracellular events using live imaging.

    Conclusions:

    • Single-cell electroporation is a powerful technique for studying individual cells in vivo.
    • The optimized SCE protocol in Xenopus tadpoles facilitates detailed analysis of neuronal function and development.
    • This method provides a valuable tool for cell-autonomous investigations in a living vertebrate model.