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Related Experiment Video

Updated: Jun 27, 2026

Murine Skin Transplantation

Murine Skin Transplantation

Published on: January 16, 2008

Murine skin transplantation.

Kym R Garrod¹, Michael D Cahalan

¹Department of Physiology and Biophysics, University of California, Irvine, USA.

Journal of Visualized Experiments : Jove

|December 11, 2008

Summary

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This video demonstrates mouse skin transplantation, a key method for studying T cell responses to foreign antigens. This technique allows researchers to assess immune reactions to MHC-disparate donors efficiently.

Area of Science:

Immunology
Surgical Techniques
Transplantation Biology

Background:

Skin transplantation is a widely used model to study host T cell responses against MHC-disparate donor antigens.
This method is considered stringent yet technically straightforward, making it valuable for immunological research.

Purpose of the Study:

To provide a detailed, step-by-step visual guide for performing skin transplantation in a mouse model.
To serve as an educational resource for researchers aiming to utilize this technique.

Main Methods:

The protocol involves five key stages: donor skin harvesting, recipient preparation, the transplantation procedure, graft monitoring post-bandage removal, and troubleshooting tips.
The procedure is designed to be completed in under 10 minutes by experienced personnel.

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Murine Full-thickness Skin Transplantation

Murine Full-thickness Skin Transplantation

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Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Published on: June 29, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Murine Skin Transplantation

Murine Skin Transplantation

Published on: January 16, 2008

Murine Full-thickness Skin Transplantation

Murine Full-thickness Skin Transplantation

Published on: January 2, 2017

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

Published on: June 29, 2022

Main Results:

Successful execution of the skin transplantation protocol allows for the assessment of host immune responses.
The video demonstrates the practical application of a standard immunological assay.

Conclusions:

Mouse skin transplantation is an accessible and effective model for investigating T cell-mediated rejection.
This visual guide facilitates the adoption and proficiency of this critical immunological technique.