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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Updated: Jun 27, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Pyrosequencing: a simple method for accurate genotyping.

Cristi King¹, Tiffany Scott-Horton

¹Department of Internal Medicine, Washington University of St. Louis, USA.

Journal of Visualized Experiments : Jove

|December 11, 2008

Summary

This summary is machine-generated.

Pyrosequencing is a simple yet thorough genotyping method for analyzing genetic polymorphisms. This technique enables rapid single-nucleotide polymorphism evaluation and is beneficial for pharmacogenetic research.

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Pyrosequencing: A Simple Method for Accurate Genotyping

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Area of Science:

Genetics
Pharmacogenetics
Molecular Biology

Background:

The Human Genome Project has generated vast amounts of genetic data.
This data explosion necessitates advanced genotyping methods for genetic analysis.

Purpose of the Study:

To explain the technique and methodology of Pyrosequencing.
To highlight Pyrosequencing as an efficient tool for pharmacogenetic research.

Main Methods:

Pyrosequencing is presented as a thorough and simple genotyping method.
The method analyzes single-nucleotide polymorphisms, tri-allelic polymorphisms, indels, and short-repeat polymorphisms.
Allele percentages for methylation or pooled samples can be determined.

Main Results:

Pyrosequencing offers rapid and efficient single-nucleotide polymorphism evaluation.
The method includes a standardized control sequence for internal quality control.
It is applicable to clinically relevant polymorphisms.

Conclusions:

Pyrosequencing is a valuable and versatile tool for genetic polymorphism analysis.
Its efficiency and thoroughness make it well-suited for pharmacogenetic applications.