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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
DNA Bacteriophages01:26

DNA Bacteriophages

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Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

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Viral Recombination00:57

Viral Recombination

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Related Experiment Video

Updated: Jun 27, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Modifying bacteriophage lambda with recombineering.

Lynn C Thomason1, Amos B Oppenheim, Donald L Court

  • 1Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 11, 2008
PubMed
Summary
This summary is machine-generated.

Recombineering is a powerful in vivo genetic engineering technique for modifying bacterial and phage genomes. This method enables precise genetic alterations like deletions and insertions using the Red recombination system.

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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

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Last Updated: Jun 27, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Area of Science:

  • Molecular Biology
  • Microbial Genetics

Background:

  • Recombineering is an advanced in vivo genetic engineering method.
  • It is applicable to Escherichia coli and other Gram-negative bacteria.

Purpose of the Study:

  • To introduce genetic modifications in various DNA molecules.
  • To demonstrate the versatility of recombineering for genome engineering.

Main Methods:

  • Utilizes the bacteriophage lambda Red generalized recombination system.
  • Catalyzes homologous recombination between linear DNA and replicons with short homology arms (50 base pairs).
  • Employs single-stranded oligonucleotides or double-stranded PCR products for direct genome modification.

Main Results:

  • Enables creation of single-base changes, small and large deletions, and small insertions.
  • Successfully applied to phage lambda, bacterial chromosomes, plasmids, and bacterial artificial chromosomes (BACs).
  • Demonstrates potential for modifying other bacteriophage genomes.

Conclusions:

  • Recombineering offers a versatile and efficient approach for in vivo genome engineering.
  • The technique facilitates precise genetic modifications in diverse DNA contexts.
  • Further applications in other bacteriophages are anticipated.