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SERS-based plasmonic nanobiosensing in single living cells.

Jonathan P Scaffidi1, Molly K Gregas, Victoria Seewaldt

  • 1Department of Biomedical Engineering, Duke University, 136 Hudson Hall, P. O. Box 90281, Durham, NC 27708, USA.

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|December 11, 2008
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We developed a pH-sensitive fiber-optic nanoprobe for intracellular bioanalysis. This surface-enhanced Raman scattering (SERS) tool enables sensitive monitoring of cellular pH in single living human cells.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Intracellular pH is a critical parameter for cellular function.
  • Existing methods for monitoring intracellular pH often lack sensitivity or specificity.
  • Advanced nanoprobes are needed for precise single-cell analysis.

Purpose of the Study:

  • To develop and apply a novel pH-sensitive plasmonics-active fiber-optic nanoprobe.
  • To utilize surface-enhanced Raman scattering (SERS) for intracellular bioanalysis.
  • To demonstrate the nanoprobe's capability for monitoring intracellular pH in living human cells.

Main Methods:

  • Fabrication of a plasmonics-active fiber-optic nanoprobe.
  • Functionalization of the nanoprobe for pH sensitivity.
  • Surface-enhanced Raman scattering (SERS) spectroscopy for detection.
  • Application in HMEC-15/hTERT and PC-3 human cell lines.

Main Results:

  • The developed nanoprobe exhibited pH sensitivity.
  • SERS detection allowed for effective intracellular pH measurements.
  • Successful monitoring of intracellular pH in both normal and cancer cell lines.
  • Demonstrated sensitivity and selectivity in cellular microenvironment analysis.

Conclusions:

  • The pH-sensitive fiber-optic nanoprobe is a viable tool for intracellular bioanalysis.
  • SERS-based nanoprobes offer a sensitive and selective method for single-cell pH monitoring.
  • This technology provides a new means to study cellular microenvironments in living cells.