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Updated: Jun 27, 2026
Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
Published on: May 9, 2020
More rapid and sensitive method for simultaneous determination of tryptophan and kynurenic acid by HPLC
Lan-gan Pi1, Ai-guo Tang, Xi-ming Mo
1Department of Clinical Laboratory, The Second Xiangya Hospital, Central South University, No.139, Renmingzhong Road, Changsha, Hunan 410011, China.
Objective:
To describe a simple, rapid, and sensitive HPLC method for simultaneous determination of TRP and KYNA in human serum.
Design And Method:
Samples were deproteinized by perchloric acid. KYNA was detected at 344 nm of excitation wavelength and 404 nm of emission wavelength, TRP was detected at 254 nm and 404 nm, with a total run time of 13 min per sample.
Results:
Standard curves of 0.49 micromol/L to 196 micromol/L of TRP were linear. Inter-day and intra-day coefficient of variations were 3.31% and 4.14%, respectively. Average recovery was 104.43%. Detection limit was 0.001 micromol/L. The linearity of the assay was maintained from 1.5 nmol/L to 2093 nmol/L of KYNA. Inter-day and intra-day CVS were 3.20% and 4.27%, respectively. Average recovery was 101.19%. Detection limit was 0.05 nmol/L.
Conclusion:
The developed HPLC method is simple, convenient and can be applied in the diagnosis of related diseases.
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