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A specific fluorogenic assay for N-acetylgalactosamine-4-sulphatase activity using immunoadsorption
D A Brooks1, G J Gibson, P A McCourt
1Department of Chemical Pathology, Adelaide Medical Centre for Women and Children, Australia.
Journal of Inherited Metabolic Disease
|January 1, 1991
Abstract:
A method combining immune capture and enzyme detection by fluorochemistry has been developed for the diagnostic assay of N-acetylgalactosamine-4-sulphatase (4-sulphatase). The procedure uses a monoclonal antibody 4-S 4.1 to immunoadsorb 4-sulphatase specifically from complex protein samples containing other sulphatases, and 4-methylumbelliferyl sulphate to detect captured 4-sulphatase. The assay provides an accurate and simple method for the diagnosis of Maroteaux-Lamy syndrome (Mucopolysaccharidosis type VI).