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Updated: Jun 27, 2026

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Published on: January 7, 2019
The Escherichia coli K5 capsule is not synthesized in a protected compartment within the cytoplasm
Thomas Hudson1, Marie Goldrick, Ian S Roberts
1University of Manchester, United Kingdom.
Abstract:
The intracellular expression of the K5 lyase enzyme, which degrades the K5 polysaccharide, decreased cell surface expression of the Escherichia coli K5 capsule. This indicates that biosynthesis of K5 polysaccharide in the cytoplasm is accessible to the action of K5 lyase and is not synthesized within a protected cytoplasmic compartment.
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