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Related Concept Videos

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

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Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Drug-Receptor Interactions

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Ligand Binding Sites02:40

Ligand Binding Sites

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Signal Transduction: Overview

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Related Experiment Video

Updated: Jun 27, 2026

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
14:05

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Published on: September 19, 2013

Receptor binding ligands to image infection.

M Chianelli1, O C Boerman, G Malviya

  • 1Department of Diagnostics, Regina Apostolorum Hospital, Albano, Rome, Italy.

Current Pharmaceutical Design
|December 17, 2008
PubMed
Summary

Researchers are exploring new radiopharmaceuticals for infection imaging beyond white blood cells. Radiolabeled antibiotics and antimicrobial peptides show promise for diagnosing infections, but further research is needed.

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Area of Science:

  • Nuclear Medicine
  • Radiopharmaceutical Chemistry
  • Infectious Disease Imaging

Background:

  • Current infection imaging relies on radiolabeled white blood cells, which have limitations in safety, simplicity, and cost.
  • A need exists for receptor-specific ligands to image infections and differentiate sterile from septic inflammation.

Purpose of the Study:

  • To review and evaluate various receptor-specific ligands as potential alternatives for infection imaging.
  • To assess the potential of radiolabeled antibodies, antibiotics, and antimicrobial peptides for infection diagnosis.

Main Methods:

  • Review of literature on various radioligands tested for infection imaging.
  • Analysis of different classes of ligands including peptides, antibodies, and antimicrobial agents.
  • Evaluation of radiolabeled antibodies, antibiotics, and antimicrobial peptides as infection-specific radiopharmaceuticals.

Main Results:

  • Radiolabeled antibodies are a viable alternative to labeled white blood cells in specific scenarios.
  • Radiolabeled antibiotics and antimicrobial peptides are promising for infection-specific imaging.
  • Further investigation into binding and accumulation mechanisms of these radiopharmaceuticals in bacteria is required.

Conclusions:

  • Radiolabeled antibodies offer a valid alternative for infection imaging under certain conditions.
  • Radiolabeled antibiotics and antimicrobial peptides represent promising avenues for developing infection-specific radiopharmaceuticals.
  • Understanding the fundamental mechanisms of radiopharmaceutical interaction with bacteria is crucial for future development.