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Recent patents on self-quenching DNA probes
Jens-Peter Knemeyer1, Nicole Marmé
1Department of Functional Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. knemeyer@single-molecule-spectroscopy.de
Recent Patents on DNA & Gene Sequences
|December 17, 2008
Summary
This review covers patents for self-quenching DNA probes used in DNA/RNA assays, including in living cells. These probes enhance fluorescence upon target binding, improving various homogeneous and surface-based assay formats.
Area of Science:
- Molecular Biology
- Biotechnology
- Analytical Chemistry
Background:
- Self-quenching DNA probes utilize fluorescence resonance energy transfer (FRET), photoinduced electron transfer (PET), or electronic interactions for signal generation.
- These probes are designed for homogeneous and surface-based assay formats, enabling sensitive detection of nucleic acids.
- Patents cover various designs, including self-quenching primers, TaqMan probes, G-quartet probes, Molecular Beacons, Smart Probes, and Pleiades Probes.
Purpose of the Study:
- To review patents related to self-quenching DNA probes for nucleic acid detection.
- To highlight the application of these probes in homogeneous and surface-based assays, including in living cells.
- To provide an overview of different self-quenching probe technologies and their mechanisms.
Main Methods:
- Review of patent literature focusing on self-quenching DNA probe technologies.
- Analysis of probe mechanisms, including fluorescence quenching and restoration upon target hybridization or degradation.
- Categorization of probes based on their design and application in various assay formats.
Main Results:
- Identification of numerous patents detailing self-quenching DNA probes for diverse applications.
- Demonstration of probe utility in amplification-based assays (e.g., PCR) and direct nucleic acid detection.
- Confirmation that most homogeneous probes are adaptable for surface-based assay improvements.
Conclusions:
- Self-quenching DNA probes represent a significant advancement in nucleic acid detection technologies.
- The reviewed patents showcase the versatility and broad applicability of these probes in diagnostics and research.
- Further development and patenting in this area are expected to yield improved assay performance and novel applications.
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