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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Sensitive bioanalysis: combining single-molecule spectroscopy with mono-labeled self-quenching probes

Nicole Marmé1, Jens-Peter Knemeyer

  • 1Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 229, Heidelberg, Germany. marme@single-molecule-spectroscopy.de

Analytical and Bioanalytical Chemistry
|June 15, 2007
PubMed
Summary

This review highlights fluorescence single-molecule spectroscopy for ultra-sensitive bioanalysis. Novel assays using self-quenching probes and photoinduced electron transfer (PET) are presented for DNA, enzyme, and antibody detection.

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