Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Incorporating tracer-tracee differences into models to improve accuracy.

D A Schoeller1

  • 1Department of Medicine, University of Chicago, Illinois.

JPEN. Journal of Parenteral and Enteral Nutrition
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circannual variation in relative weight of children 5 to 16 years of age.

Pediatric obesity·2018
Same author

δ<sup>13</sup>C values of an herbivore and the ratio of C<sub>3</sub> to C<sub>4</sub> plant carbon in its diet.

Oecologia·2017
Same author

High energy expenditure is not protective against increased adiposity in children.

Pediatric obesity·2016
Same author

Response to 'Energy balance measurement: when something is not better than nothing'.

International journal of obesity (2005)·2015
Same author

Familial and individual predictors of obesity and insulin resistance in urban Hispanic children.

Pediatric obesity·2015
Same author

Breath carbon stable isotope ratios identify changes in energy balance and substrate utilization in humans.

International journal of obesity (2005)·2014

Isotopically labeled compounds are ideal metabolic tracers, but subtle metabolic differences can affect kinetic data accuracy. The doubly labeled water method highlights how tracer-tracee metabolism variations impact energy expenditure calculations.

Area of Science:

  • Biomedical research
  • Metabolic studies
  • Isotope tracing

Background:

  • Ideal tracers mimic tracee behavior for accurate metabolic studies.
  • Isotopically labeled compounds offer near-identical chemical properties to tracees.
  • Minor metabolic differences introduced by isotopic substitution can be significant.

Purpose of the Study:

  • To evaluate the impact of tracer-tracee metabolic discrepancies on kinetic data.
  • To analyze how isotopic substitution affects the accuracy of metabolic measurements.
  • To illustrate these challenges using the doubly labeled water method.

Main Methods:

  • Review of tracer principles in metabolic research.
  • Analysis of kinetic data interpretation in isotope studies.

Related Experiment Videos

  • Case study: Doubly labeled water method for energy expenditure.

    • Main Results:

    • Isotopic substitution, while minimal, can alter metabolic pathways.
    • These alterations can quantitatively affect the interpretation of kinetic data.
    • The doubly labeled water method's accuracy is sensitive to these metabolic differences.

    Conclusions:

    • Careful consideration of tracer-tracee metabolic differences is crucial for accurate kinetic modeling.
    • Mathematical models must account for potential isotopic effects.
    • Ensuring tracer behavior closely matches tracee metabolism is key for reliable metabolic studies.